SONATA BIS
“Studies on the use of Lab-on-a-chip systems to analyze the regeneration of heart cells.”

NCN SONATA BIS, 2019/34/E/ST5/00381, 2020 – 2024

Funding: National Science Centre, Poland

Grant period: 31.03.2020-29.11.2024

Project description:

It is known, that cardiovascular (CVDs) diseases are the most common causes of death around the world. The main challenge to treat CVDs is the fact that the damaged cardiomyocytes (CMs) do not renew. However, the newest research indicates potential of stem cells (SCs) to regenerate heart tissue. To define proper parameters for cell regeneration, SC differentiation towards heart cells should be studied under conditions as close as possible to native tissue. Conventional culture models do not fulfill such requirements. Perfusion Lab-on-a-chip systems, mimicking heart tissue features, seem to be a suitable tool used for optimization parameters of heart cell regeneration. Although, Heart-on -a- chip systems used to cardiac cell culture and study drug cardiotoxicity have been developed, some additional features of heart (which enhance cardiac cell maturation) are often skipped.

The goal of the proposed Project is to study how parameters such as perfusion, nanofibers, stem cells and biochemical factors influence regeneration of heart cells under disease (hypoxia) state. For this purpose, a new Lab-on-a-chip system will be developed and integrated with additional biomaterials (a new fabricated nanofibrous mats), which should enhance cardiac cell maturation and stem cells differentiation towards cardiac cells. The geometry of the microsystem will be optimized and designed in such a way that both mono- and cocultures of cardiac and stem cells will be performed simultaneously.

       

Team members:

  • Principal Investigator: D.Sc. Ph.D. Eng. prof. PW Elżbieta Jastrzębska
  • Co-investigator: Ph.D. Ewelina Krogulec (Nencki Institute of Experimental Biology PAS)
  • Co-investigator: Ph.D. Eng. Michał Wojasiński, M.Sc. Eng. Iwona Łopianiak (Faculty of Chemical and Process Engineering, Warsaw University of Technology)
  • Co-investigator: Ph.D. Eng. Patrycja Sokołowska (Baranowska) , Ph.D. Eng. Kamil Żukowski
  • PhD students: M.Sc. Eng. Dominik Kołodziejek, M.Sc. Eng. Zuzanna Iwoń
  • Students: Eng. Oliwia Tadko, Eng. Aleksandra Kierlańczyk

Publications:

  1. Kołodziejek D., Sierańska U., Iwoń Z., Łopianiak I., Krogulec E., Wojasiński M., Jastrzębska E.: Cardiac tissue modeling using flow microsystems and nanofiber mats: Evaluating hypoxia-induced cellular and molecular changes, Sensors and Actuators B – Chemical, 403, 2024, 135169, 135169_1-135169_11, DOI:10.1016/j.snb.2023.135169
  2. Iwon Z., Kierlanczyk A., Sokolowska P., Wojasinki M., Lopianiak I., Krogulec E., Jastrzebska E., Improving rodents and humans cardiac cell maturity in vitro through polycaprolactone and polyurethane nanofibers, Biomedical Materials 2024, 19(2), 025031,DOI: 10.1088/1748-605X/ad240a
  3. Kołodziejek D., Łopianiak I., Tadko O., Drozd M., Wojasiński M., Jastrzębska E.: Magnetic polyurethane nanomaterials: A novel approach for in vitro cardiac cell maturation and culture, Polymer Testing, 2023, vol. 127, pp.1-9, Article number:108190. DOI:10.1016/j.polymertesting.2023.108190
  4. Kobuszewska A., Kołodziejek D., Wojasiński M., Jastrzębska E., Ciach T., Brzózka Z.: Lab-on-a-chip system integrated with nanofiber mats used as a potential tool to study cardiovascular diseases (CVDs), Sensors and Actuators B – Chemical, 2021, vol. 330, pp.1-12, Article number:129291. DOI:10.1016/j.snb.2020.12929
  5. Kobuszewska A., Kołodziejek D., Wojasiński M., Ciach T., Brzózka Z., Jastrzębska E.: Study of stem cells influence on cardiac cells cultured with a cyanide-p-trifluoromethoxyphenylhydrazone in Organ-on-a-Chip system, Biosensors, 2021, vol. 11, no. 5, pp.1-14, Article number:131. DOI:10.3390/bios1105013
  6. Kobuszewska A., Jastrzębska E., Żukowski K., Brzózka Z.: Simulation of hypoxia of myocardial cells in microfluidic systems, Scientific Reports, 2020, vol. 10, no. 1, pp.1-11, Article number:15524. DOI:10.1038/s41598-020-72660-w
  7. Iwoń Z., Krogulec E., M. Wojasiński, E. Jastrzębska, Hypoxia and re-oxygenation effects on human cardiomyocytes cultured on polycaprolactone and polyurethane nanofibrous mats – Journal of Biological Engineering, 18, 2024, 37, DOI: 10.1186/s13036-024-00432-5
  8. Iwoń Z., Krogulec E., Tarnowska I., Łopianiak I., M. Wojasiński, Dobrzyn A., Jastrzębska E., Maturation of human cardiomyocytes derived from induced pluripotent stem cells (iPSC- CMs) on polycaprolactone and polyurethane nanofibrous mats, Scietific reports 14, 2024, 12975, DOI:10.1038/s41598-024-63905-z
  9. Kołodziejek D., Sierańska U., Iwoń Z., Łopianiak I., Krogulec E., Wojasiński M., Jastrzębska E.: Cardiac tissue modeling using flow microsystems and nanofiber mats: Evaluating hypoxia-induced cellular and molecular changes, Sensors and Actuators B – Chemical, 403, 2024, 135169, 135169_1-135169_11, DOI:10.1016/j.snb.2023.135169
  10. Iwon Z., Kierlanczyk A., Sokolowska P., Wojasinki M., Lopianiak I., Krogulec E., Jastrzebska E., Improving rodents and humans cardiac cell maturity in vitro through polycaprolactone and polyurethane nanofibers, Biomedical Materials 2024, 19(2), 025031,DOI: 10.1088/1748-605X/ad240a
  11. Patent application: Dominik Kołodziejek, Elżbieta Jastrzębska, Marcin Drozd, Michał Wojasiński, Iwona Łopianiak, Materiały nanowłókniste magnetyczne, sposób ich wytwarzania oraz zastosowanie, No. app: P.443235, 22.12.2022

Project dissemination:

  1. Zuzanna Iwon, Dominik Kolodziejek, Inez Tarnowska, Urszula Sierańska, Ewelina Krogulec, Michal Wojasinski, Iwona Lopianiak, Elżbieta Jastrzebska, Advancing Cardiac Cell Modeling: From Nanofiber Scaffolds to Integrated Microsystems, NanoBio Tech, 12-15.11.2023, Montreux, Switzerland, oral presentation
  2. Oliwia Tadko, Dominik Kołodziejek, Marcin Drozd, Michał Wojasiński, Iwona Łopianiak, Elżbieta Jastrzębska, A Novel Microfluidic System Integrated with Magnetic Nanofibers for Human Heart Modelling, I Ogólnopolska Konferencja NanoBioTechMedyczna Nanobiomedica, 21-22.09.2023, Warsaw, Poland, poster
  3. Dominik Kołodziejek, Oliwia Tadko, Marcin Drozd, Michał Wojasiński, Iwona Łopianiak, Elżbieta Jastrzębska, Novel Lab-on-a-chip System: Mechanical Stimulation Of Cardiac Cells Via Magnetic Nanofibers And Alternating Magnetic Fields, MicroTAS 2023, 15-19.10.2023, Katowice, Poland, oral presentation
  4. Dominik Kołodziejek, Urszula Sierańska, Michał Wojasiński, Iwona Łopianiak, Marcin Drozd, Tomasz Ciach, Elżbieta Jastrzębska. Nanofibrous mats integrated with PC-PDMS-PC microsystem for hypoxia studies in cardiac cells. 10th Intercollegiate Biotechnology Symposium „Symbioza”. 12-14.05.2023, Warsaw, Poland, oral presentation
  5. Oliwia Tadko, Dominik Kołodziejek, Zuzanna Żółtowska, Natalia Wasiak, Marcin Drozd, Elżbieta Jastrzębska, Novel Magnetic Culture Substrates: Polycarbonate and Polydimethylsiloxane Membranes for Studying Cardiac Cell Responses, MicroTAS 2023, 15-19.10.2023, Katowice, Poland, poster
  6. Zuzanna Iwoń, Michał Wojasiński, Inez Tarnowska, Ewelina Krogulec, Elżbieta Jastrzębska, The nanofibrous mats for human cardiac cells culture,Xth Intercollegiate Biotechnology Symposium “SymbiozaXI, 12-14.05.2023, poster
  7. Zuzanna Iwoń, Aleksandra Kierlanczyk, Ewelina Krogulec, Michal Wojasinski, Iwona Lopianiak, Elzbieta Jastrzebska, Nanofibers with different physicochemical properties as a new substrate for micro- and nanoengineering used for hypoxia simulation human cardiomyocytes, MicroTAS 2023, 15-19.10.2023, 15-19.10.2023, Katowice, Poland, poster
  8. Zuzanna Iwoń, Iwona Łopianiak, Patrycja Sokołowska, Ewelina Krogulec, Tomasz Ciach, Elżbieta Jastrzębska, Badanie zastosowania nanowłókien polimerowych w hodowli ludzkich kardiomiocytów, XI Polska Konferencja Chemii Analitycznej, PoKoChA 2022 (Łódź, 19-23.06.2022), poster
  9. Zuzanna Iwon, Michal Wojasinski, Iwona Lopianiak, Aleksandra Kierlanczyk, Pawel Dobrzyn, Tomasz Ciach, Elzbieta Jastrzebska, Study of the application of polymer nanofibers in a cardiac cellular model in vitro, 10th European Young Engineers Conference, 04-06.04.2022, Warsaw, Poland, oral presentation
  10. Dominik Kołodziejek, Oliwia Tadko, Michal Wojasinski, Iwona Lopianiak, Marcin Drozd, Tomasz Ciach, Elzbieta Jastrzebska, Novel nanofiber material with magnetic properties for cardiac cel cultures. 26th Internaional Conference on Miniaturized Systems for Chemistry and Life Science, Hangzhou, China, on-line, 23-27.10.2022, poster
  11. Dominik Kołodziejek, Urszula Sierańska, Michal Wojasinski, Iwona Lopianiak, Zbigniew Brzózka, Elzbieta Jastrzebska, Hypoxia simulation in PC-PDMS-PC microsystem integrated with nanofibrous mats. 26th Internaional Conference on Miniaturized Systems for Chemistry and Life Science, Hangzhou, China, on-line, 23-27.10.2022, poster
  12. Dominik Kołodziejek, Oliwia Tadko, Michal Wojasinski, Iwona Lopianiak, Marcin Drozd, Tomasz Ciach, Elzbieta Jastrzebska, Novel magnetic nanofibers for cardiac cell grown and maturation 10th European Young Engineers Conference, Warszawa, 04-06.04.2022, oral presentation
  13. Dominik Kolodziejek, Michal Wojasinski, Iwona Lopianiak, Zuzanna Iwon, Elzbieta Jastrzebska; „Heart-on-a-chip system with polymeric nanofiber mats as a new approach for mimicking heart tissue microphysiology”, Organoids and Organs-on-Chips Europe 2022, 21-22. 06. 2022 – Rotterdam, Niderlands, oral presentation
  14. Zuzanna Iwoń , Dominik Kołodziejek , Michał Wojasiński , Iwona Łopianiak , Patrycja Sokołowska , Ewelina Krogulec , Elżbieta Jastrzebska, Micro- and nano-technology approach to study cardiac cell function,prezentacja, NanoBio Tech, 14-16.11, Montreux, Switzerland, poster
  15. Kobuszewska, D. Kołodziejek, M. Wojasiński, T. Ciach, Z. Brzózka, E. Jastrzębska, „Lab-on-a-Chip system integrated with nanofiber mats for biochemical simulation of hypoxia of cardiac cells”, 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020, 4-9.10.2020, on-line, poster
  16. Zuzannoa Iwoń, Michał Wojasiński, Tomasz Ciach, Paweł Dobrzyń, Elżbieta Jastrzębska „Badanie żywotności i ukierunkowania szczurzych kardiomioblastów hodowanych na nanowłóknach
    z polikaprolatonu” XVII Warszawskim Seminarium Doktorantów Chemików Chemsession 2021, 24.09.2021, poster
  17. Dominik Kołodziejek, Michał Wojasiński, Katarzyna  Kociszewska, Elżbieta Jastrzębska „ Trójwarstwowy mikrosystem przepływowy wykonany z PC-PDMS-PC z ukierunkowanymi matami nanowłóknistymi do badania stanów chorobowych oraz regeneracji komórek mięśnia serca”. XVII Warszawskim Seminarium Doktorantów Chemików Chemsession 2021, 24.09.2021, poster
  18. Dominik Kolodziejek, Michał  Wojasinski, Katarzyna Kociszewska, Kamil Zukowski, Zbigniwe Brzozka, Elżbieta Jastrzebska „ New PC-PDMS-PC microsystem with aligned nanofibers mats to study heart disease”, 25 th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, 10-14.10.2021 – Palm Springs/hybrydowo, poster