NCBiR LIDER, LIDER/026/573/L-4/12/NCBR/2013

Funding: National Centre for Research and Development

National Science Centre, Poland

LIDER Programme – the purpose of the program is to expand the competence of young scientists in independently planning, managing, and leading their own research teams while carrying out projects that have the potential for implementation in the economy.

Grant period: 01.01.2014-17.01.2026

Project description:

The goal of the project is to develop and fabricate a new microsystem used to culture and model the growth of cardiac muscle cells. The main task is to develop the geometry of the microcircuit in such a way that the conditions for cell culture are as close as possible to those in vivo. In addition, it is planned to create suitable scaffolds in the microchannels of the system to enable spatial arrangement and growth of myocardial cells. For this purpose, biomaterials used in classical tissue engineering (e.g., hydrogels) will be used.

Team members:

• Principal Investigator: Ph.D. Eng. Elżbieta Jastrzębska
• Co-investigator: Ph.D. Eng. Iwona Lasocka (Warsaw University of Life Sciences)
• Co-investigator: Ph.D. Eng. Kamil Żukowski, Ph.D. Eng. Ilona Grabowska-Jadach
• PhD students/Students: M.Sc. Eng. Ewelina Tomecka, M.Sc. Anna Kobuszewska, M.Sc

Publications:

1. Tomecka E., Jastrzębska E., Chudy M., Dybko A.: Research on the use of hydrogel for the three-dimensional cell culture in microfluidic system, Proceedings of SPIE: The International Society for Optical Engineering , 2014, vol. 9291, pp.92910K1-92910K7. DOI:10.1117/12.2073354
2. Jastrzębska E., Tomecka E., Jesion I.: Heart-on-a-chip based on stem cell biology, Biosensors & Bioelectronics, 2016, vol. 75, pp.67-81. DOI:10.1016/j.bios.2015.08.012
3. Kobuszewska A., Tomecka E., Jastrzębska E., Żukowski K., Chudy M., Renaud P., Dybko A., Brzózka Z.: Heart-on-a-Chip: An Investigation of the Influence of Static and Perfusion Conditions on Cardiac (H9C2) Cell Proliferation, Morphology, and Alignment, SLAS Technology, 2017, vol. 22, no. 5, pp.536-546. DOI:10.1177/2472630317705610
4. Tomecka E., Wojasiński M., Jastrzębska E., Chudy M., Ciach T., Brzózka Z.: Poly(l-lactic acid) and polyurethane nanofibers fabricated by solution blow spinning as potential substrates for cardiac cell culture, Materials Science & Engineering C – Materials for Biological Applications, 2017, vol. 75, pp.305-316. DOI:10.1016/j.msec.2017.02.055
5. Żuchowska A., Baranowska P., Flont M., Brzózka Z., Jastrzębska E., 3D cell-on-a-chip models in bioanalytical science, Analytica Chimica Acta, review, 342413,doi.org/10.1016/j.aca.2024.342413